13-18 May 2018
Casino Conference Centre
Europe/Prague timezone

Hydroxyapatite nanoparticles labelled with 18F

17 May 2018, 17:30
1h 30m
Gallery (Casino Conference Centre)

Gallery

Casino Conference Centre

Reitenbergerova 4/95, Mariánské Lázně, Czech Republic
Poster Radiopharmaceutical Chemistry, Labelled Compounds Poster RPH

Speaker

Ekaterina Kukleva (Czech Technical University in Prague, Faculty of Nuclear Sciences and Physical Engineering)

Description

Hydroxyapatite based materials including nanoparticles (HAp-NPs) are widely used in medicine because of their stability in various biological media [1]. The HAp-NPs also belong to promising drug carrier systems for medicinal radionuclides such as F-18 as the part of multipurpose theranostic system with other therapeutic and diagnostic radionuclides [2]. HAp-NPs can show uptake by cancer tissue due to EPR effect, therefore diagnostic and therapy of the diseases are expected to be very accurate and beneficial.
Fluorine-18 was obtained in a form of irradiated water for medicinal purposes and was used without further purification. The labelling was performed with ready-made HAp-NPs (5 mg) in 0.9% NaCl solution. Labelling was carried out with pristine NPs and NPs decorated with selected ligands: NPMIDA and IDMP (0.05 mg). Experiments with pristine HAp-NPs have shown F-18 uptake with maximal yield of about 93% (15 min incubation), while NPMIDA-HAp-NPs and IDMP-HAp-NPs have shown yields 99% and 98% respectively. Further experiments have shown that uptake kinetics is quite fast (over 90% yield within 5 minutes). In vitro stability experiments have shown good stability of 18F-HAp-NPs nanoconstruct in a bovine blood serum and plasma (more than 90% of activity remained bound to the NPs in 7,5 hours), and slightly worth results in 5% albumin solution and physiological saline (under 90%). NPMIDA and IDMP decorated nanoparticles have shown good stability in a physiological saline (over 95% of bound activity in 6 hours), between 65 – 80% in serum and 40 – 50% in 5% albumin solution.
Acknowledgment: AZV ČR - 16-30544A.

References
[1] E. M. Rivera-Munoz, in Biomedical Engineering – Frontiers and Challenges, (Ed: R. Fazel), Rijeka, Croatia, 2011, pp. 75-98.
[2] B. Sandhöfer, M. Meckel et al, Appl. Mater. Interfaces. 2015; 7, 19.

Primary authors

Ekaterina Kukleva (Czech Technical University in Prague, Faculty of Nuclear Sciences and Physical Engineering) Martin Vlk (Department of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, CTU Prague) Mr Ján Kozempel (Czech Technical University in Prague, Faculty of Nuclear Sciences and Physical Engineering, Department of Nuclear Chemistry)

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