Dr
Stanislav Pavelka
(Dept. of Radiometry, Inst. of Physiolgy, ASCR Prague; and Inst. of Biochemistry, Masaryk Univ. Brno, Czech Rep.)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
With the use of adapted radiometric enzyme assays for iodothyronine sulfotransferases (ST) and uridine 5’-diphospho-glucuronyltransferase (UDPGT), as well as of our newly developed radiometric assays for iodothyronine deiodinases (IDs) of types 1, 2 and 3 (D1, D2 and D3), we studied the interaction of an antidepressant drug fluoxetine with the metabolism of thyroid hormones (TH) in the rat. TH...
Mr
Bahrom Yarmatov
(Institute Nuclear Physics of academy of sciences of Uzbekistan)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
For labeling of pharmacological preparation of isoniazide we have used the method of labeling by thermally activated tritium. Isoniazide was labeled by thermally activated tritium in apparatus for tritium labeling. The influence of procedure of labeling on isoniazide has been investigated. During of labeling the change of color of isoniazide was observed that indicates partial destruction of...
Mr
Jakhongir Dadakhonov
(Institute Nuclear Physics of academy of sciences of Uzbekistan)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
For labeling of pharmacological preparations of furosemide and drotaverine we used the method of labeling by thermally activated tritium. Furosemide and drotaverine were labeled by thermally activated tritium in apparatus for tritium labeling. The optimum regime of labeling was selected. The systems of purification of tritium-labeled furosemide and drotaverine by thin layer chromatography...
Mrs
Teija Koivula
(M.Sc., researcher)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
The crude reaction mixture consisted of p-[18F]MPPF and 2-4 labelled impurities eluting after the product fraction, and the reverse-phase HPLC method reported in the literature [1] failed sometimes to separate p-[18F]MPPF from its radioactive by-product with close retention time. Analytical HPLC was based on similar chromatographic conditions. By comparing it to...
Dr
Dennis Wester
(MDS Nordion)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
Both 67Ga (t1/2 3.26 d) and 68Ga (t1/2 68 min) are radioisotopes with appropriate emissions for nuclear imaging. 68Ga is of particular interest due to its potential availability from a 68Ge/68Ga generator. The short half-life of 68Ga requires the use of targeting vectors with fast localization such as small...
Dr
Maria Chernysheva
(Lomonosov Moscow State University)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
The behavior of biological active compounds in aqueous/oil systems is usually used as a model of their interaction with the lipid membrane of the cells. Thus, study of the processes proceeding in the system of two immiscible liquids is a central question of the research. In this work we propose to use tritium labeled bioactive compounds and scintillation phase method [1] for studying...
Dr
Martin Kropacek
(Nuclear Physics Institute of the ASCR, 25068 Rez by Prague, Czech Republic)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
Lutetium-177 is a radionuclide used in nuclear medicine for therapeutic application. Both β and γ radiations emitted from 177Lu allow imaging of the therapeutic radionuclide without need of adding any other radionuclide. It can be prepared in the nuclear reactor via direct (n, γ) activation from enriched lutetium target or via indirect neutron activation from ytterbium oxide. In...
Ms
Veronika Kleinova
(Nuclear Physics Institute of the ASCR, 250 68 Rez by Prague, Czech Republic)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
The ability of the monoclonal antibody TU-20 and its scFv fragment to bind specifically to the C-end of the class III β-tubulin makes these substances useful as potential diagnostics for neurodegenerative diseases – especially peripheral neuropathies.
TU-20 and its scFv were labeled with 125I and 123I by chloramine-T (with average yield 0.75 and 0.50, respectively). Radiochemical purity...
Dr
Stanislav Pavelka
(Dept. of Radiometry, Inst. of Physiolgy, ASCR Prague; and Inst. of Biochemistry, Masaryk Univ. Brno, Czech Rep.)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
We elaborated novel, reliable methods for extremely sensitive radiometric determination of enzyme activities of iodothyronine deiodinases (IDs) of types 1, 2 and 3 in microsomal fractions of different rat and human tissues, as well as in homogenates of cultured mammalian cells. These enzymes catalyze selective 5’- (outer ring) and 5- (inner ring) monodeiodinations of iodothyronines and play...
Ms
Supaporn Sawadjoon
(National Cyclotron and PET Centre, Chulabhorn Cancer Centre, Vipavadee-Rangsit Highway, Laksi, Bangkok 10210, Thailand)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
6-[18F]Fluoro-3,4-dihydroxyphenyl-L-alanine (6-[18F]fluoro-L-DOPA) is an analog of L-DOPA and it is used for studying metabolism of dopamine function in humans with Positron Emission Tomography. Therefore, the need for reliable and simple production of this tracer had led to the improvement of the routine production. Various electrophilic and nucleophilic methods for the...
Ms
Maria Mayordomo Martinez
(Department of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, CTU Prague)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
Triterpenes are a group of natural compounds. They are studied for their wide spectrum of biological activities: antitumor, antimutagenic, antiinflammatory, antiallergy, endocrine, immunomodulatory, antiviral, hepatoprotective, cardioprotective, antithrombotic or activities on central nervous system.1
Nowadays the investigation of antitumor drugs is really important because over one million...
Ms
Marie Reková
(student)
4/22/10, 12:00 PM
Nuclear Methods in Medicine, Radiopharmaceuticals and Radiodiagnostics, Labelled Compounds
Poster
Model study of the γ-immunoglobulin G (Human or Bovine IgG, polyclonal antibodies) and bifunctional chelating agent diethylenetriaminepentaacetic acid dianhydride (cDTPAA) conjugation was carried out. Various values of the cDTPAA/antibody conjugation ratio (15/1, 40/1, 105/1, 125/1 and 250/1) and the weight concentration of IgG 10, 5 and 1 mg mL-1 in 0.1 mol L-1 phosphate...
